血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯免疫吸附試驗法)

ELISA Kit for Serum Amyloid A (SAA)

SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

  • 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
  • SEA885Bo.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測血清淀粉樣蛋白A(SAA),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的血清淀粉樣蛋白A(SAA)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 88-101 96
EDTA plasma(n=5) 86-105 92
heparin plasma(n=5) 86-97 93

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的血清淀粉樣蛋白A(SAA),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中血清淀粉樣蛋白A(SAA)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 81-102% 81-103% 91-102%
EDTA plasma(n=5) 90-98% 81-90% 98-105% 88-104%
heparin plasma(n=5) 78-92% 81-97% 85-99% 79-97%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數。

實驗原理

將血清淀粉樣蛋白A(SAA)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的血清淀粉樣蛋白A(SAA)與連接于固相載體上的抗體結合,然后加入生物素化的血清淀粉樣蛋白A(SAA)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血清淀粉樣蛋白A(SAA)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號 適用物種:Bos taurus; Bovine (Cattle,牛) 應用(僅供研究使用,不用于臨床診斷!)
RPA885Bo01 血清淀粉樣蛋白A(SAA)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA885Bo01 血清淀粉樣蛋白A(SAA)多克隆抗體 WB; IHC; ICC; IP.
MAA885Bo21 血清淀粉樣蛋白A(SAA)單克隆抗體 WB; IHC; ICC; IP.
SEA885Bo 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.

參考文獻

雜志 參考文獻
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US National Library of Medicine National Institutes of Health No association between vitamin D levels and inflammation markers in patients with acute coronary syndrome [Pubmed:25615956]
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121]
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Journal of?Dairy?Science Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows [Pubmed: 17430930]
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Journal of Animal Science Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465]
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